nhp ifnγ elispot kit  (R&D Systems)

Journal: Communications Biology

Article Title: ChAdOx1 nCoV-19 protection against SARS-CoV-2 in rhesus macaque and ferret challenge models

doi: 10.1038/s42003-021-02443-0

Figure Lengend Snippet: Spike-specific T cell response in rhesus macaques ( a ) and ferrets ( b ) monitored by IFNγ ELISpot following vaccination and ICS (ferrets only). Response from ChAdOx1 nCoV-19 vaccinated NHPs was analysed with a Friedman one-way anova and post hoc test. Response in ferrets was analysed with a non-parametric one-way anova (Kruskal−Wallis) and post hoc Dunn’s multiple comparison test. A significant increase in the response compared to Day 0 was observed from day 14 onwards, with no statistically significant increase in the T cell response following booster vaccination. T cell responses in ferrets were measured by intracellular cytokine staining on day 28 post-vaccination and compared to responses measured by IFNγ ELISpot.

Article Snippet: IFNγ ELISpot assay was performed using NHP IFNγ (Mabtech) or Ferret IFNγ ELISpot BASIC Kit according to the manufacturer’s protocol (MABtech).

Techniques: Enzyme-linked Immunospot, Comparison, Staining

Journal: Communications Biology

Article Title: ChAdOx1 nCoV-19 protection against SARS-CoV-2 in rhesus macaque and ferret challenge models

doi: 10.1038/s42003-021-02443-0

Figure Lengend Snippet: a Immune responses following challenge of rhesus macaques with SARS-CoV-2 were measured in virus neutralisation assays and by IFNγ ELISpot. b Quantification of CD4 + and CD8 + T cells expressing HLA-DR and PD-1 prior to (day 0) and at days 3, 6−7 (7) and 13−14 post SARS-CoV-2 challenge of NHPs. Data in each graph was analysed with a two-way analysis of variance (repeated measure) and a post hoc Tukey test, p values indicate a significant difference ( p < 0.05) within vaccine groups over time. c Quantification of NHP monocyte subpopulations determined by the expression of CD14 and CD16 by whole blood immunophenotyping flow cytometry assay. Bars show group medians with values measured in individual animals shown. Data in each graph was analysed with a two-way analysis of variance (repeated measure) and a post hoc Tukey test, p values indicate a significant difference ( p < 0.05) within vaccine groups over time. d Antibody responses in ferrets following challenge were measured in the virus neutralisation assay. e To determine whether antibody responses impacted on the protection of ferrets from SARS-CoV-2 infection, a Pearson correlation analysis was performed comparing peak viraemia in each ferret to IgG ELISA Unit, neutralisation titre (PRNT 50 ), psuedoneutralisation titre (mVNT), or IFNγ ELISpot on the day of challenge, r 2 and p values are indicated on each graph.

Article Snippet: IFNγ ELISpot assay was performed using NHP IFNγ (Mabtech) or Ferret IFNγ ELISpot BASIC Kit according to the manufacturer’s protocol (MABtech).

Techniques: Virus, Enzyme-linked Immunospot, Expressing, Flow Cytometry, Infection, Enzyme-linked Immunosorbent Assay

Journal: PLoS Pathogens

Article Title: Antibody Quality and Protection from Lethal Ebola Virus Challenge in Nonhuman Primates Immunized with Rabies Virus Based Bivalent Vaccine

doi: 10.1371/journal.ppat.1003389

Figure Lengend Snippet: ELISPOT GP response on frozen cells (spots per million cells).

Article Snippet: To evaluate T- cell responses to EBOV GP, NHP PBMCs were tested using the NHP IFNγ ELISPOT Kit (R&D Systems, Cat#EL961) as per the manufacturer's instructions.

Techniques: Enzyme-linked Immunospot